Part:BBa_K200005:Design
OtsA: Part 1 of 2 for trehalose producing enzymes.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 383
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR from Escherichia Coli using BL21(DE3) as the genomic DNA template and the Pfu Ultra II enzyme. Primers were designed to include overhangs coding for XbaI and SpeI recognition sites in order to allow the gene to be BioBricked according to the BioBrick Standard. The primers are as follows:
Forward PCR primer containing XbaI overhang (bold) for BioBricking: GCTCTAGATGAGTCGTTTAGTCGTAG
Recomended Temperatures for PCR : 52.4C(without overhang) and 63.2(with overhang)
Reverse PCR primer containing SpeI overhang (bold) for BioBricking: GGACTAGTACTACGCAAGCTTTGGAAAG
Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang)
BioBrick overhang shown in bold
Source
Escherichia coli BL21(DE3)